LAPSE:2021.0305
Published Article
LAPSE:2021.0305
NMPC-Based Workflow for Simultaneous Process and Model Development Applied to a Fed-Batch Process for Recombinant C. glutamicum
Philipp Levermann, Fabian Freiberger, Uma Katha, Henning Zaun, Johannes Möller, Volker C. Hass, Karl Michael Schoop, Jürgen Kuballa, Ralf Pörtner
April 30, 2021
For the fast and improved development of bioprocesses, new strategies are required where both strain and process development are performed in parallel. Here, a workflow based on a Nonlinear Model Predictive Control (NMPC) algorithm is described for the model-assisted development of biotechnological processes. By using the NMPC algorithm, the process is designed with respect to a target function (product yield, biomass concentration) with a drastically decreased number of experiments. A workflow for the usage of the NMPC algorithm as a process development tool is outlined. The NMPC algorithm is capable of improving various process states, such as product yield and biomass concentration. It uses on-line and at-line data and controls and optimizes the process by model-based process extrapolation. In this study, the algorithm is applied to a Corynebacterium glutamicum process. In conclusion, the potency of the NMPC algorithm as a powerful tool for process development is demonstrated. In particular, the benefits of the system regarding the characterization and optimization of a fed-batch process are outlined. With the NMPC algorithm, process development can be run simultaneously to strain development, resulting in a shortened time to market for novel products.
Keywords
C. glutamicum, digitalization, model-based process development, NMPC algorithm, process modeling, process optimization
Suggested Citation
Levermann P, Freiberger F, Katha U, Zaun H, Möller J, Hass VC, Schoop KM, Kuballa J, Pörtner R. NMPC-Based Workflow for Simultaneous Process and Model Development Applied to a Fed-Batch Process for Recombinant C. glutamicum. (2021). LAPSE:2021.0305
Author Affiliations
Levermann P: Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, 21073 Hamburg, Germany
Freiberger F: Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, 21073 Hamburg, Germany
Katha U: GALAB Laboratories GmbH, 21029 Hamburg, Germany
Zaun H: GALAB Laboratories GmbH, 21029 Hamburg, Germany
Möller J: Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, 21073 Hamburg, Germany [ORCID]
Hass VC: Faculty Medical and Life Sciences, Hochschule Furtwangen University, 78120 Furtwangen, Germany
Schoop KM: Ingenieurbüro Dr.-Ing. Schoop GmbH, 21109 Hamburg, Germany
Kuballa J: GALAB Laboratories GmbH, 21029 Hamburg, Germany [ORCID]
Pörtner R: Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, 21073 Hamburg, Germany [ORCID]
Journal Name
Processes
Volume
8
Issue
10
Article Number
E1313
Year
2020
Publication Date
2020-10-19
Published Version
ISSN
2227-9717
Version Comments
Original Submission
Other Meta
PII: pr8101313, Publication Type: Journal Article
Record Map
Published Article

LAPSE:2021.0305
This Record
External Link

doi:10.3390/pr8101313
Publisher Version
Download
Files
[Download 1v1.pdf] (3.7 MB)
Apr 30, 2021
Main Article
License
CC BY 4.0
Meta
Record Statistics
Record Views
370
Version History
[v1] (Original Submission)
Apr 30, 2021
 
Verified by curator on
Apr 30, 2021
This Version Number
v1
Citations
Most Recent
This Version
URL Here
https://psecommunity.org/LAPSE:2021.0305
 
Original Submitter
Calvin Tsay
Links to Related Works
Directly Related to This Work
Publisher Version