LAPSE:2021.0277
Published Article
LAPSE:2021.0277
Microbial Communities and Sulfate-Reducing Microorganisms Abundance and Diversity in Municipal Anaerobic Sewage Sludge Digesters from a Wastewater Treatment Plant (Marrakech, Morocco)
April 29, 2021
Abstract
Both molecular analyses and culture-dependent isolation were combined to investigate the diversity of sulfate-reducing prokaryotes and explore their role in sulfides production in full-scale anaerobic digesters (Marrakech, Morocco). At global scale, using 16S rRNA gene sequencing, Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Synergistetes, and Euryarchaeota were the most dominant phyla. The abundance of Archaea (3.1−5.7%) was linked with temperature. The mcrA gene ranged from 2.18 × 105 to 1.47 × 107 gene copies.g−1 of sludge. The sulfate-reducing prokaryotes, representing 5% of total sequences, involved in sulfides production were Peptococcaceae, Syntrophaceae, Desulfobulbaceae, Desulfovibrionaceae, Syntrophobacteraceae, Desulfurellaceae, and Desulfobacteraceae. Furthermore, dsrB gene ranged from 2.18 × 105 to 1.92 × 107 gene copies.g−1 of sludge. The results revealed that exploration of diversity and function of sulfate-reducing bacteria may play a key role in decreasing sulfide production, an undesirable by-product, during anaerobic digestion.
Both molecular analyses and culture-dependent isolation were combined to investigate the diversity of sulfate-reducing prokaryotes and explore their role in sulfides production in full-scale anaerobic digesters (Marrakech, Morocco). At global scale, using 16S rRNA gene sequencing, Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Synergistetes, and Euryarchaeota were the most dominant phyla. The abundance of Archaea (3.1−5.7%) was linked with temperature. The mcrA gene ranged from 2.18 × 105 to 1.47 × 107 gene copies.g−1 of sludge. The sulfate-reducing prokaryotes, representing 5% of total sequences, involved in sulfides production were Peptococcaceae, Syntrophaceae, Desulfobulbaceae, Desulfovibrionaceae, Syntrophobacteraceae, Desulfurellaceae, and Desulfobacteraceae. Furthermore, dsrB gene ranged from 2.18 × 105 to 1.92 × 107 gene copies.g−1 of sludge. The results revealed that exploration of diversity and function of sulfate-reducing bacteria may play a key role in decreasing sulfide production, an undesirable by-product, during anaerobic digestion.
Record ID
Keywords
anaerobic digestion, Archaea, biogas, microbial diversity, sulfate-reducing bacteria, sulfides
Subject
Suggested Citation
El Houari A, Ranchou-Peyruse M, Ranchou-Peyruse A, Bennisse R, Bouterfas R, Goni Urriza MS, Qatibi AI, Guyoneaud R. Microbial Communities and Sulfate-Reducing Microorganisms Abundance and Diversity in Municipal Anaerobic Sewage Sludge Digesters from a Wastewater Treatment Plant (Marrakech, Morocco). (2021). LAPSE:2021.0277
Author Affiliations
El Houari A: Environmental Microbiology, Université de Pau et des Pays de l’Adour, E2S UPPA, CNRS, IPREM UMR 5254, 64000 Pau, France; Water Biodiversity and Climate Change Laboratory, Semlalia-Sciences Faculty, Cadi Ayyad University, 40 000 Marrakech, Morocco [ORCID]
Ranchou-Peyruse M: Environmental Microbiology, Université de Pau et des Pays de l’Adour, E2S UPPA, CNRS, IPREM UMR 5254, 64000 Pau, France
Ranchou-Peyruse A: Environmental Microbiology, Université de Pau et des Pays de l’Adour, E2S UPPA, CNRS, IPREM UMR 5254, 64000 Pau, France [ORCID]
Bennisse R: Water Biodiversity and Climate Change Laboratory, Semlalia-Sciences Faculty, Cadi Ayyad University, 40 000 Marrakech, Morocco
Bouterfas R: Water Biodiversity and Climate Change Laboratory, Semlalia-Sciences Faculty, Cadi Ayyad University, 40 000 Marrakech, Morocco
Goni Urriza MS: Environmental Microbiology, Université de Pau et des Pays de l’Adour, E2S UPPA, CNRS, IPREM UMR 5254, 64000 Pau, France
Qatibi AI: Water Biodiversity and Climate Change Laboratory, Semlalia-Sciences Faculty, Cadi Ayyad University, 40 000 Marrakech, Morocco
Guyoneaud R: Environmental Microbiology, Université de Pau et des Pays de l’Adour, E2S UPPA, CNRS, IPREM UMR 5254, 64000 Pau, France [ORCID]
Ranchou-Peyruse M: Environmental Microbiology, Université de Pau et des Pays de l’Adour, E2S UPPA, CNRS, IPREM UMR 5254, 64000 Pau, France
Ranchou-Peyruse A: Environmental Microbiology, Université de Pau et des Pays de l’Adour, E2S UPPA, CNRS, IPREM UMR 5254, 64000 Pau, France [ORCID]
Bennisse R: Water Biodiversity and Climate Change Laboratory, Semlalia-Sciences Faculty, Cadi Ayyad University, 40 000 Marrakech, Morocco
Bouterfas R: Water Biodiversity and Climate Change Laboratory, Semlalia-Sciences Faculty, Cadi Ayyad University, 40 000 Marrakech, Morocco
Goni Urriza MS: Environmental Microbiology, Université de Pau et des Pays de l’Adour, E2S UPPA, CNRS, IPREM UMR 5254, 64000 Pau, France
Qatibi AI: Water Biodiversity and Climate Change Laboratory, Semlalia-Sciences Faculty, Cadi Ayyad University, 40 000 Marrakech, Morocco
Guyoneaud R: Environmental Microbiology, Université de Pau et des Pays de l’Adour, E2S UPPA, CNRS, IPREM UMR 5254, 64000 Pau, France [ORCID]
Journal Name
Processes
Volume
8
Issue
10
Article Number
E1284
Year
2020
Publication Date
2020-10-14
ISSN
2227-9717
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PII: pr8101284, Publication Type: Journal Article
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LAPSE:2021.0277
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https://doi.org/10.3390/pr8101284
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Apr 29, 2021
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Calvin Tsay
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