LAPSE:2021.0102
Published Article
LAPSE:2021.0102
Development, Characterization and In Vitro Evaluation of Paclitaxel and Anastrozole Co-Loaded Liposome
Minh Thanh Vu, Dinh Tien Dung Nguyen, Ngoc Hoi Nguyen, Van Thu Le, The Nam Dao, Thi Huong Nguyen, Tien Dung Cong, Truc Le-Buu Pham, Tri Duc Lam, Ngoc Thuy Trang Le
March 14, 2021
Paclitaxel (PTX) and anastrozole (ANA) have been frequently applied in breast cancer treatment. PTX is well-known for its anti-proliferative effect meanwhile ANA has just been discovered to act as an estrogen receptor α (ERα) ligand. The combination therapy of PTX and ANA is expected to improve treating efficiency, as ANA would act as a ligand binding with the ERα gene expressed in breast cancer cells and thereafter PTX would inhibit the division and cause death to those cancer cells. In this study, liposome-based nanocarriers (LP) were developed for co-encapsulation of PTX and ANA to improve the efficacy of the combined drugs in an Estrogen receptor-responsive breast cancer study. PTX-ANA co-loaded LP was prepared using thin lipid film hydration method and was characterized for morphology, size, zeta potential, drug encapsulation and in vitro drug release. In addition, cell proliferation (WST assay) and IN Cell Analyzer were used for in vitro cytotoxicity studies on a human breast cancer cell line (MCF-7). Results showed that the prepared LP and PTX-ANA-LP had spherical vesicles, with a mean particle size of 170.1 ± 13.5 nm and 189.0 ± 22.1 nm, respectively. Controlled and sustained releases were achieved at 72 h for both of the loaded drugs. The in vitro cytotoxicity study found that the combined drugs showed higher toxicity than each single drug separately. These results suggested a new approach to breast cancer treatment, consisting of the combination therapy of PTX and ANA in liposomes based on ER response.
Keywords
anastrozole, co-loaded, drug delivery system, paclitaxel, soy lecithin liposome
Subject
Suggested Citation
Vu MT, Nguyen DTD, Nguyen NH, Le VT, Dao TN, Nguyen TH, Cong TD, Pham TLB, Lam TD, Le NTT. Development, Characterization and In Vitro Evaluation of Paclitaxel and Anastrozole Co-Loaded Liposome. (2021). LAPSE:2021.0102
Author Affiliations
Vu MT: Institute for Chemistry and Materials, 17 Hoang Sam, Cau Giay, Hanoi City 100000, Vietnam [ORCID]
Nguyen DTD: Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi City 100000, Vietnam; Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City 700000, Vietnam
Nguyen NH: Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City 700000, Vietnam [ORCID]
Le VT: Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City 700000, Vietnam [ORCID]
Dao TN: Institute for Chemistry and Materials, 17 Hoang Sam, Cau Giay, Hanoi City 100000, Vietnam
Nguyen TH: Institute for Chemistry and Materials, 17 Hoang Sam, Cau Giay, Hanoi City 100000, Vietnam [ORCID]
Cong TD: Hanoi University of Mining and Geology, 18 Pho Vien, Duc Thang, Tu Liem, Hanoi City 100000, Vietnam [ORCID]
Pham TLB: Biotechnology Center of Ho Chi Minh City, 2374, Highway 1, Trung My Tay Ward, District 12, Ho Chi Minh City 700000, Vietnam
Lam TD: NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam
Le NTT: Institute of Research and Development, Duy Tan University, Danang 550000, Vietnam [ORCID]
Journal Name
Processes
Volume
8
Issue
9
Article Number
E1110
Year
2020
Publication Date
2020-09-06
Published Version
ISSN
2227-9717
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Original Submission
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PII: pr8091110, Publication Type: Journal Article
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LAPSE:2021.0102
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doi:10.3390/pr8091110
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Mar 14, 2021
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CC BY 4.0
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Mar 14, 2021
 
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Original Submitter
Calvin Tsay
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