LAPSE:2021.0073v1
Published Article
LAPSE:2021.0073v1
A Multi-Scale Approach to Modeling the Interfacial Reaction Kinetics of Lipases with Emphasis on Enzyme Adsorption at Water-Oil Interfaces
Sherly Rusli, Janna Grabowski, Anja Drews, Matthias Kraume
February 22, 2021
The enzymatic hydrolysis of triglycerides with lipases (EC 3.1.1.3.) involves substrates from both water and oil phases, with the enzyme molecules adsorbed at the water-oil (w/o) interface. The reaction rate depends on lipase concentration at the interface and the available interfacial area in the emulsion. In emulsions with large drops, the reaction rate is limited by the surface area. This effect must be taken into account while modelling the reaction. However, determination of the interfacial saturation is not a trivial matter, as enzyme molecules have the tendency to unfold on the interface, and form multi-layer, rendering many enzyme molecules unavailable for the reaction. A multi-scale approach is needed to determine the saturation concentration with specific interfacial area so that it can be extrapolated to droplet swarms. This work explicitly highlights the correlation between interfacial adsorption and reaction kinetics, by integration of the adsorption kinetics into the enzymatic reaction. The rate constants were fitted globally against data from both single droplet and drop swarm experiments. The amount of adsorbed enzymes on the interface was measured in a single drop with a certain surface area, and the enzyme interfacial loading was estimated by Langmuir adsorption isotherm.
Keywords
enzymatic hydrolysis, interfacial kinetics modeling, lipases, protein adsorption
Suggested Citation
Rusli S, Grabowski J, Drews A, Kraume M. A Multi-Scale Approach to Modeling the Interfacial Reaction Kinetics of Lipases with Emphasis on Enzyme Adsorption at Water-Oil Interfaces. (2021). LAPSE:2021.0073v1
Author Affiliations
Rusli S: Chair of Chemical and Process Engineering, Technical University of Berlin, Ackerstrasse 76, 13355 Berlin, Germany [ORCID]
Grabowski J: Chair of Chemical and Process Engineering, Technical University of Berlin, Ackerstrasse 76, 13355 Berlin, Germany
Drews A: HTW Berlin, Department of Engineering II, School of Life Science Engineering, Wilhelminenhofstr. 75A, 12459 Berlin, Germany
Kraume M: Chair of Chemical and Process Engineering, Technical University of Berlin, Ackerstrasse 76, 13355 Berlin, Germany [ORCID]
Journal Name
Processes
Volume
8
Issue
9
Article Number
E1082
Year
2020
Publication Date
2020-09-02
Published Version
ISSN
2227-9717
Version Comments
Original Submission
Other Meta
PII: pr8091082, Publication Type: Journal Article
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LAPSE:2021.0073v1
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doi:10.3390/pr8091082
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Feb 22, 2021
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CC BY 4.0
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[v1] (Original Submission)
Feb 22, 2021
 
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Feb 22, 2021
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Calvin Tsay
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