LAPSE:2020.0950
Published Article
LAPSE:2020.0950
The Evolution of Cell Free Biomanufacturing
August 29, 2020
Cell-free systems are a widely used research tool in systems and synthetic biology and a promising platform for manufacturing of proteins and chemicals. In the past, cell-free biology was primarily used to better understand fundamental biochemical processes. Notably, E. coli cell-free extracts were used in the 1960s to decipher the sequencing of the genetic code. Since then, the transcription and translation capabilities of cell-free systems have been repeatedly optimized to improve energy efficiency and product yield. Today, cell-free systems, in combination with the rise of synthetic biology, have taken on a new role as a promising technology for just-in-time manufacturing of therapeutically important biologics and high-value small molecules. They have also been implemented at an industrial scale for the production of antibodies and cytokines. In this review, we discuss the evolution of cell-free technologies, in particular advancements in extract preparation, cell-free protein synthesis, and cell-free metabolic engineering applications. We then conclude with a discussion of the mathematical modeling of cell-free systems. Mathematical modeling of cell-free processes could be critical to addressing performance bottlenecks and estimating the costs of cell-free manufactured products.
Keywords
cell-free protein synthesis, cell-free systems, synthetic biology
Subject
Suggested Citation
Vilkhovoy M, Adhikari A, Vadhin S, Varner JD. The Evolution of Cell Free Biomanufacturing. (2020). LAPSE:2020.0950
Author Affiliations
Vilkhovoy M: Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14850, USA [ORCID]
Adhikari A: Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14850, USA [ORCID]
Vadhin S: Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14850, USA [ORCID]
Varner JD: Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14850, USA [ORCID]
Journal Name
Processes
Volume
8
Issue
6
Article Number
E675
Year
2020
Publication Date
2020-06-08
Published Version
ISSN
2227-9717
Version Comments
Original Submission
Other Meta
PII: pr8060675, Publication Type: Review
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LAPSE:2020.0950
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doi:10.3390/pr8060675
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Aug 29, 2020
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CC BY 4.0
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Aug 29, 2020
 
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Aug 29, 2020
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Original Submitter
Calvin Tsay
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