LAPSE:2020.0281
Published Article
LAPSE:2020.0281
Curcumin Prevents Cyclophosphamide-Induced Lung Injury in Rats by Suppressing Oxidative Stress and Apoptosis
March 12, 2020
Abstract
Curcumin (CUR) has been used since ancient times to treat several ailments as it possesses many pharmacological activities. This study intended to explore the mechanism underlying the protective effects of CUR in remodeling oxidative stress and apoptotic signals in cyclophosphamide (CP)-induced pulmonary injury in albino rats. CUR was administered at a dose of 300 mg/kg/day for 7 days and on the seventh day a single dose of CP (200 mg/kg) was given. Histopathological and ultrastructural examinations of CP-intoxicated rats showed complete alveolar obstruction, thickened inter-alveolar septa, enlarged blood vessels, severe inflammatory edema with pyknotic nuclei, and disappearance of cytoplasmic organelles. Significant increases in caspase-3, malondialdehyde (MDA), and protein carbonyl (PCO) and significant decreases in superoxide dismutase (SOD) and glutathione peroxidase (GPx) were observed. In contrast, rats that received CUR showed clear and empty lumina with single row of pneumocytes, disappearance of edema, and no interstitial electron dense bodies in rats’ lung tissues. Additionally, CUR significantly reduced caspase-3, MDA, and PCO and increased SOD and GPx. In conclusion, these findings revealed the protective effects of CUR against CP-induced pulmonary injury in rats through suppressing oxidative damage and apoptosis.
Curcumin (CUR) has been used since ancient times to treat several ailments as it possesses many pharmacological activities. This study intended to explore the mechanism underlying the protective effects of CUR in remodeling oxidative stress and apoptotic signals in cyclophosphamide (CP)-induced pulmonary injury in albino rats. CUR was administered at a dose of 300 mg/kg/day for 7 days and on the seventh day a single dose of CP (200 mg/kg) was given. Histopathological and ultrastructural examinations of CP-intoxicated rats showed complete alveolar obstruction, thickened inter-alveolar septa, enlarged blood vessels, severe inflammatory edema with pyknotic nuclei, and disappearance of cytoplasmic organelles. Significant increases in caspase-3, malondialdehyde (MDA), and protein carbonyl (PCO) and significant decreases in superoxide dismutase (SOD) and glutathione peroxidase (GPx) were observed. In contrast, rats that received CUR showed clear and empty lumina with single row of pneumocytes, disappearance of edema, and no interstitial electron dense bodies in rats’ lung tissues. Additionally, CUR significantly reduced caspase-3, MDA, and PCO and increased SOD and GPx. In conclusion, these findings revealed the protective effects of CUR against CP-induced pulmonary injury in rats through suppressing oxidative damage and apoptosis.
Record ID
Keywords
antioxidant, apoptosis, curcumin, cyclophosphamide, protein carbonyl, pulmonary injury
Subject
Suggested Citation
Saghir SAM, Alharbi SA, Al-Garadi MA, Al-Gabri N, Rady HY, Olama NK, Abdulghani MAM, Al Hroob AM, Almaiman AA, Bin-Jumah M, Mahmoud AM, Taha M. Curcumin Prevents Cyclophosphamide-Induced Lung Injury in Rats by Suppressing Oxidative Stress and Apoptosis. (2020). LAPSE:2020.0281
Author Affiliations
Saghir SAM: Department of Medical Analysis, Princess Aisha Bint Al-Hussein College of Nursing and Medical Sciences, Al-Hussein Bin Talal University, Ma’an 71111, Jordan; Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penan [ORCID]
Alharbi SA: Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
Al-Garadi MA: Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia [ORCID]
Al-Gabri N: Department of Pathology, Faculty of Veterinary Medicine, Thamar University, Dhamar 2153, Yemen; Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt [ORCID]
Rady HY: Department of Anatomy and Embryology, Faculty of Medicine, Ain Shams University, Cairo 11517, Egypt
Olama NK: Department of Anatomy and Embryology, Faculty of Medicine, Ain Shams University, Cairo 11517, Egypt
Abdulghani MAM: Department of Pharmacology & Toxicology, Unaizah College of Pharmacy, Qassim University, Al Qassim 51911, Saudi Arabia
Al Hroob AM: Department of Medical Analysis, Princess Aisha Bint Al-Hussein College of Nursing and Medical Sciences, Al-Hussein Bin Talal University, Ma’an 71111, Jordan
Almaiman AA: Department of Applied Medical Sciences, Community College of Unaizah, Qassim University, Buraydah 51431, Saudi Arabia
Bin-Jumah M: Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh 84428, Saudi Arabia
Mahmoud AM: Department of Zoology, Physiology Division, Faculty of Science, Beni-Suef University, Beni-Suef 62514, Egypt [ORCID]
Taha M: Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt
Alharbi SA: Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
Al-Garadi MA: Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia [ORCID]
Al-Gabri N: Department of Pathology, Faculty of Veterinary Medicine, Thamar University, Dhamar 2153, Yemen; Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt [ORCID]
Rady HY: Department of Anatomy and Embryology, Faculty of Medicine, Ain Shams University, Cairo 11517, Egypt
Olama NK: Department of Anatomy and Embryology, Faculty of Medicine, Ain Shams University, Cairo 11517, Egypt
Abdulghani MAM: Department of Pharmacology & Toxicology, Unaizah College of Pharmacy, Qassim University, Al Qassim 51911, Saudi Arabia
Al Hroob AM: Department of Medical Analysis, Princess Aisha Bint Al-Hussein College of Nursing and Medical Sciences, Al-Hussein Bin Talal University, Ma’an 71111, Jordan
Almaiman AA: Department of Applied Medical Sciences, Community College of Unaizah, Qassim University, Buraydah 51431, Saudi Arabia
Bin-Jumah M: Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh 84428, Saudi Arabia
Mahmoud AM: Department of Zoology, Physiology Division, Faculty of Science, Beni-Suef University, Beni-Suef 62514, Egypt [ORCID]
Taha M: Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt
Journal Name
Processes
Volume
8
Issue
2
Article Number
E127
Year
2020
Publication Date
2020-01-21
ISSN
2227-9717
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Original Submission
Other Meta
PII: pr8020127, Publication Type: Journal Article
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LAPSE:2020.0281
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https://doi.org/10.3390/pr8020127
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[v1] (Original Submission)
Mar 12, 2020
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Mar 12, 2020
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Record Owner
Calvin Tsay
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