LAPSE:2019.1636
Published Article
LAPSE:2019.1636
Inhibition of Key Enzymes Linked to Obesity and Cytotoxic Activities of Whole Plant Extracts of Vernonia mesplilfolia Less
December 16, 2019
The whole plant of Vernonia mespilifolia is widely used as a traditional remedy for obesity in South Africa. The aim of this study was to investigate the anti-obesity and cytotoxic effects of Vernonia mespilifolia extracts in vitro. The α-amylase, α-glucosidase, and lipase inhibitory activities of aqueous and ethanol extracts of Vernonia mespilifolia were investigated, while the cytotoxic effects of these extracts were analyzed using Hoechst 33342 and propidium iodide (PI) dual staining on a human cervical HeLa cell line. The results showed that the LC50 (the concentration of a material will kill 50% of test organisms) values of aqueous and ethanol extracts of Vernonia mespilifolia were >200 and 149 µg/mL, respectively, to HeLa cells. Additionally, the ethanol extract exhibited the strongest inhibitory effect on the pancreatic lipase (Half-maximal inhibitory concentration (IC50) = 331.16 µg/mL) and on α-amylase (IC50 = 781.72 µg/mL), while the aqueous extract has the strongest α-glucosidase (IC50 = 450.88 µg/mL). Our results suggest that Vernonia mespilifolia’s acclaimed anti-obesity effects could be ascribed to its ability to inhibit both carbohydrate and fat digesting enzymes.
Keywords
HeLa, Hoechst 33342/PI staining, lipase, Vernonia mespilifolia, α-amylase, α-glucosidase
Subject
Suggested Citation
Unuofin JO, Otunola GA, Afolayan AJ. Inhibition of Key Enzymes Linked to Obesity and Cytotoxic Activities of Whole Plant Extracts of Vernonia mesplilfolia Less. (2019). LAPSE:2019.1636
Author Affiliations
Unuofin JO: Department of Life and Consumer Sciences, University of South Africa, Cnr Christiaan de Wet and Pioneer Ave, Private Bag X6, Florida 1710, South Africa; Department of Botany, Medicinal Plants and Economic Development (MPED) Research Centre, University of [ORCID]
Otunola GA: Department of Botany, Medicinal Plants and Economic Development (MPED) Research Centre, University of Fort Hare, Alice 5700, South Africa [ORCID]
Afolayan AJ: Department of Botany, Medicinal Plants and Economic Development (MPED) Research Centre, University of Fort Hare, Alice 5700, South Africa
Journal Name
Processes
Volume
7
Issue
11
Article Number
E841
Year
2019
Publication Date
2019-11-10
Published Version
ISSN
2227-9717
Version Comments
Original Submission
Other Meta
PII: pr7110841, Publication Type: Journal Article
Record Map
Published Article

LAPSE:2019.1636
This Record
External Link

doi:10.3390/pr7110841
Publisher Version
Download
Files
Dec 16, 2019
Main Article
License
CC BY 4.0
Meta
Record Statistics
Record Views
626
Version History
[v1] (Original Submission)
Dec 16, 2019
 
Verified by curator on
Dec 16, 2019
This Version Number
v1
Citations
Most Recent
This Version
URL Here
https://psecommunity.org/LAPSE:2019.1636
 
Original Submitter
Calvin Tsay
Links to Related Works
Directly Related to This Work
Publisher Version