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Records with Keyword: Fermentation
Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology
Abdi Hanra Sebayang, Masjuki Haji Hassan, Hwai Chyuan Ong, Surya Dharma, Arridina Susan Silitonga, Fitranto Kusumo, Teuku Meurah Indra Mahlia, Aditiya Harjon Bahar
March 26, 2019 (v1)
Subject: Biosystems
Keywords: alternative fuel, bioethanol, Fermentation, hydrolysis, Manihot glaziovii (M. glaziovii), Optimization
Bioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was performed with selected hydrolysis parameters, including substrate loading, stroke speed, α-amylase concentration and amyloglucosidase concentration. From the experiment, the resulting optimum conditions are 23.88% (w/v) substrate loading, 109.43 U/g α-amylase concentration, 65.44 U/mL amyloglucosidase concentration and 74.87 rpm stroke speed, which yielded 196.23 g/L reducing sugar. The fermentation process was also carried out, with a production value of 0.45 g ethanol/g reducing sugar, which is equivalent to 88.61% of ethanol yield after fermentation by using Saccharomyces cerevisiae (S. cerevisiae). The physical and chemical properties of the produced ethanol are within the specifications... [more]
Techno-economic comparison of Acetone-Butanol-Ethanol fermentation using various extractants
Giancarlo Dalle Ave, Thomas A. Adams II
June 12, 2018 (v1)
Keywords: Acetone, Butanol, Cost of CO2 Emissions Avoided, Ethanol, Extraction, Fermentation, Technoeconomic Analysis
This work compares various chemicals for use as extractants in second-generation Acetone-Butanol-Ethanol fermentation on economic and environmental bases. Both non-toxic and toxic extractants are considered in this study. The combinative extractive-distillation separation process was modelled using a combination of Microsoft Excel 2013, MATLAB 2015 and Aspen Plus v8.8. Separation trains were designed and optimized for each extractant to best take advantage of extractant properties. Upstream units considered in this analysis include: biomass (switchgrass) solids processing, biomass pre-treatment and saccharification, and fermentation. Downstream processes considered include utility generation and wastewater treatment. The cost of CO2 equivalent emissions avoided (CCA) was used as the metric to compare the environmental impact of each process as compared to conventional petroleum-based gasoline. The economic and environmental best extractant is shown to be 2-ethyl-hexanol with a minimum... [more]
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