Abstract
Biomacromolecules have intricate crystallisation behaviour due to their size and many interactions in solution and can often only crystallise in narrow ranges of experimental conditions. High solute concentrations are needed for crystal nucleation and growth, exceeding those eluted upstream and therefore preventing the adoption of crystallisation in downstream separation steps. By promoting molecular aggregation and nucleation via a lowered energy barrier, heterogeneous surfaces or templates can relax the supersaturation requirements and widen the crystallisation operating space. Though templates are promising candidates for process optimisation, their experimental testing has generally been limited to small-volume experiments, and quantification of their impact on process intensification and quality metrics at higher volumes remains unexplored. To address the knowledge gap, a model-based investigation of template-induced protein crystallisation systems through evaluation of key metrics is presented. Porous silica nano-particles with three chemical functionalisations (hydroxyl, carboxyl and butyl) are added to batch lysozyme crystallisation experiments at 40ml. Crystallisation population balance models are parametrised with an experimentally-validated parameter estimation methodology and further experiments are simulated. The templates appear to lower the estimated interfacial energy compared to the homogeneous case, leading to nucleation rate profiles which are less dependent on supersaturation. For this reason, the templates can crystallize quicker than the homogeneous system, particularly at lower initial concentrations. The simulation results highlight the ability of heteronucleants to alter nucleation rate profiles, and their potential to be used as process optimisation and intensification tools for biomacromolecule purification.