LAPSE:2022.0066
Published Article
LAPSE:2022.0066
Multi-Enzyme Systems in Flow Chemistry
October 13, 2022
Recent years have witnessed a growing interest in the use of biocatalysts in flow reactors. This merging combines the high selectivity and mild operation conditions typical of biocatalysis with enhanced mass transfer and resource efficiency associated to flow chemistry. Additionally, it provides a sound environment to emulate Nature by mimicking metabolic pathways in living cells and to produce goods through the systematic organization of enzymes towards efficient cascade reactions. Moreover, by enabling the combination of enzymes from different hosts, this approach paves the way for novel pathways. The present review aims to present recent developments within the scope of flow chemistry involving multi-enzymatic cascade reactions. The types of reactors used are briefly addressed. Immobilization methodologies and strategies for the application of the immobilized biocatalysts are presented and discussed. Key aspects related to the use of whole cells in flow chemistry are presented. The combination of chemocatalysis and biocatalysis is also addressed and relevant aspects are highlighted. Challenges faced in the transition from microscale to industrial scale are presented and discussed.
Keywords
immobilization, microreactor, reaction cascade, scale-up, whole cell
Subject
Suggested Citation
Fernandes P, de Carvalho CCCR. Multi-Enzyme Systems in Flow Chemistry. (2022). LAPSE:2022.0066
Author Affiliations
Fernandes P: Faculty of Engineering, Universidade Lusófona, 1749-024 Lisboa, Portugal; DREAMS, Universidade Lusófona, 1749-024 Lisboa, Portugal; Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisbon, Portugal; iBB—Insti [ORCID]
de Carvalho CCCR: Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisbon, Portugal; iBB—Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisbon, Portugal [ORCID]
Journal Name
Processes
Volume
9
Issue
2
First Page
225
Year
2021
Publication Date
2021-01-25
Published Version
ISSN
2227-9717
Version Comments
Original Submission
Other Meta
PII: pr9020225, Publication Type: Review
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LAPSE:2022.0066
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doi:10.3390/pr9020225
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Oct 13, 2022
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CC BY 4.0
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[v1] (Original Submission)
Oct 13, 2022
 
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Oct 13, 2022
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https://psecommunity.org/LAPSE:2022.0066
 
Original Submitter
Mina Naeini
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