LAPSE:2021.0772
Published Article
LAPSE:2021.0772
Green Synthesis of Copper Oxide Nanoparticles Using Protein Fractions from an Aqueous Extract of Brown Algae Macrocystis pyrifera
Karla Araya-Castro, Tzu-Chiao Chao, Benjamín Durán-Vinet, Carla Cisternas, Gustavo Ciudad, Olga Rubilar
October 14, 2021
Amongst different living organisms studied as potential candidates for the green synthesis of copper nanoparticles, algal biomass is presented as a novel and easy-to-handle method. However, the role of specific biomolecules and their contribution as reductant and capping agents has not yet been described. This contribution reports a green synthesis method to obtain copper oxide nanoparticles (CuO-NPs) using separated protein fractions from an aqueous extract of brown algae Macrocystis pyrifera through size exclusion chromatography (HPLC-SEC). Proteins were detected by a UV/VIS diode array, time-based fraction collection was carried out, and each collected fraction was used to evaluate the synthesis of CuO-NPs. The characterization of CuO-NPs was evaluated by Dynamic Light Scattering (DLS), Z-potential, Fourier Transform Infrared (FTIR), Transmission Electron Microscope (TEM) equipped with Energy Dispersive X-ray Spectroscopy (EDS) detector. Low Molecular Weight (LMW) and High Molecular Weight (HMW) protein fractions were able to synthesize spherical CuO-NPs. TEM images showed that the metallic core present in the observed samples ranged from 2 to 50 nm in diameter, with spherical nanostructures present in all containing protein samples. FTIR measurements showed functional groups from proteins having a pivotal role in the reduction and stabilization of the nanoparticles. The highly negative zeta potential average values from obtained nanoparticles suggest high stability, expanding the range of possible applications. This facile and novel protein-assisted method for the green synthesis of CuO-NPs may also provide a suitable tool to synthesize other nanoparticles that have different application areas.
Keywords
brown seaweed, copper oxide nanoparticles, green synthesis, proteins, size exclusion chromatography
Subject
Suggested Citation
Araya-Castro K, Chao TC, Durán-Vinet B, Cisternas C, Ciudad G, Rubilar O. Green Synthesis of Copper Oxide Nanoparticles Using Protein Fractions from an Aqueous Extract of Brown Algae Macrocystis pyrifera. (2021). LAPSE:2021.0772
Author Affiliations
Araya-Castro K: Doctoral Program in Science of Natural Resources, Universidad de La Frontera, Temuco 54-D, Chile; Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco 54-D, Chile [ORCID]
Chao TC: Institute of Environmental Change & Society, Department of Biology, University of Regina, Regina, SK S4S, Canada
Durán-Vinet B: Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco 54-D, Chile [ORCID]
Cisternas C: Doctoral Program in Science of Natural Resources, Universidad de La Frontera, Temuco 54-D, Chile
Ciudad G: Chemical Engineering Department, Universidad de La Frontera, Temuco 54-D, Chile
Rubilar O: Chemical Engineering Department, Universidad de La Frontera, Temuco 54-D, Chile
Journal Name
Processes
Volume
9
Issue
1
First Page
pr9010078
Year
2020
Publication Date
2020-12-31
Published Version
ISSN
2227-9717
Version Comments
Original Submission
Other Meta
PII: pr9010078, Publication Type: Journal Article
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LAPSE:2021.0772
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doi:10.3390/pr9010078
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Oct 14, 2021
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CC BY 4.0
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Oct 14, 2021
 
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Oct 14, 2021
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Original Submitter
Calvin Tsay
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