Encapsulation of Lactoferrin for Sustained Release Using Particles from Gas-Saturated Solutions

Kento Ono; Hiroki Sakai; Shinichi Tokunaga; Tanjina Sharmin; Taku Michael Aida; Kenji Mishima

LAPSE

Living Archive for Process Systems Engineering

LAPSE:2021.0767

Published Article

LAPSE:2021.0767

Encapsulation of Lactoferrin for Sustained Release Using Particles from Gas-Saturated Solutions

Kento Ono, Hiroki Sakai, Shinichi Tokunaga, Tanjina Sharmin, Taku Michael Aida, Kenji Mishima

October 14, 2021

The particles from gas saturated solutions (PGSS) process were performed to encapsulate lactofer-rin, an iron-binding milk glycoprotein, using supercritical carbon dioxide (scCO2). A natural en-teric polymer, shellac, was used as a coating material of lactoferrin carried out by the PGSS pro-cess. Conditions were optimized by applying different temperatures (20−50 °C) and pressures (8−10 MPa) and the particles were evaluated for particle shape and size, lactoferrin encapsulation ef-ficiency, Fourier transform infrared (FTIR) spectroscopy to confirm lactoferrin entrapment and in vitro dissolution studies at different pH values. Particles with an average diameter of 75.5 ± 7 μm were produced with encapsulation efficiency up to 71 ± 2%. Furthermore, particles that showed high stability in low pH (pH 1.2) and a sustained release over time (t2h = 75%) in higher pH (pH 7.4) suggested an effective encapsulation process for the protection of lactoferrin from gastric di-gestion.

Record ID

LAPSE:2021.0767

Keywords

enteric polymer, gastric digestion, lactoferrin, PGSS, shellac

Subject

Biosystems

Suggested Citation

Ono K, Sakai H, Tokunaga S, Sharmin T, Aida TM, Mishima K. Encapsulation of Lactoferrin for Sustained Release Using Particles from Gas-Saturated Solutions. (2021). LAPSE:2021.0767

Author Affiliations

Ono K: Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, 8-19-1 Nanakuma Jonan-ku, Fukuoka 814-0180, Japan
Sakai H: Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, 8-19-1 Nanakuma Jonan-ku, Fukuoka 814-0180, Japan
Tokunaga S: Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, 8-19-1 Nanakuma Jonan-ku, Fukuoka 814-0180, Japan
Sharmin T: Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, 8-19-1 Nanakuma Jonan-ku, Fukuoka 814-0180, Japan; Research Institute of Composite Materials, Fukuoka University, 8-19-1 Nanakuma Jonan-ku, Fukuoka 814-0180, Japan
Aida TM: Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, 8-19-1 Nanakuma Jonan-ku, Fukuoka 814-0180, Japan; Research Institute of Composite Materials, Fukuoka University, 8-19-1 Nanakuma Jonan-ku, Fukuoka 814-0180, Japan
Mishima K: Department of Chemical Engineering, Faculty of Engineering, Fukuoka University, 8-19-1 Nanakuma Jonan-ku, Fukuoka 814-0180, Japan; Research Institute of Composite Materials, Fukuoka University, 8-19-1 Nanakuma Jonan-ku, Fukuoka 814-0180, Japan

Journal Name

Processes

Volume

9

Issue

1

First Page

pr9010073

Year

2020

Publication Date

2020-12-31