LAPSE:2021.0709
Published Article
LAPSE:2021.0709
Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation
September 16, 2021
Candida antarctica Lipase B (CALB) has a wide range of applications in many fields. In this study, Pichia pastoris was used to express CALB for fermentation tank culture. Sodium dodecyl sulfate (SDS) was used as a surfactant, and foam separation technology was used to explore the best experimental conditions for the harvest of CALB. The results showed that the optimal technological conditions for the foam separation and recovery of CALB were as follows: liquid volume was 150 mL, separating gas velocity was 600 mL/min, pH value was 7, and surfactant SDS concentration was 0.5 mg/mL. Under these conditions, the enrichment ratio of CALB was 0.95, and recovery rate R was 80.32%, respectively, indicating that the foam separation technology is feasible to extract lipase B.
Record ID
Keywords
enrichment ratio, Fermentation, foam separation, lipase, recovery rate, surfactant
Subject
Suggested Citation
Shao W, Lin Y, Lu Y. Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation. (2021). LAPSE:2021.0709
Author Affiliations
Shao W: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
Lin Y: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
Lu Y: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China [ORCID]
Lin Y: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
Lu Y: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China [ORCID]
Journal Name
Processes
Volume
9
Issue
1
First Page
pr9010014
Year
2020
Publication Date
2020-12-23
Published Version
ISSN
2227-9717
Version Comments
Original Submission
Other Meta
PII: pr9010014, Publication Type: Journal Article
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Published Article
LAPSE:2021.0709
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External Link
doi:10.3390/pr9010014
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336
Version History
[v1] (Original Submission)
Sep 16, 2021
Verified by curator on
Sep 16, 2021
This Version Number
v1
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URL Here
https://psecommunity.org/LAPSE:2021.0709
Original Submitter
Calvin Tsay
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