LAPSE:2021.0375v1
Published Article
LAPSE:2021.0375v1
Determination of Dissolved CO2 Concentration in Culture Media: Evaluation of pH Value and Mathematical Data
Amir Izzuddin Adnan, Mei Yin Ong, Saifuddin Nomanbhay, Pau Loke Show
May 17, 2021
Carbon dioxide is the most influential gas in greenhouse gasses and its amount in the atmosphere reached 412 µmol/mol in August 2020, which increased rapidly, by 48%, from preindustrial levels. A brand-new chemical industry, namely organic chemistry and catalysis science, must be developed with carbon dioxide (CO2) as the source of carbon. Nowadays, many techniques are available for controlling and removing carbon dioxide in different chemical processes. Since the utilization of CO2 as feedstock for a chemical commodity is of relevance today, this study will focus on how to increase CO2 solubility in culture media used for growing microbes. In this work, the CO2 solubility in a different medium was investigated. Sodium hydroxide (NaOH) and monoethanolamine (MEA) were added to the culture media (3.0 g/L dipotassium phosphate (K2HPO4), 0.2 g/L magnesium chloride (MgCl2), 0.2 g/L calcium chloride (CaCl2), and 1.0 g/L sodium chloride (NaCl)) for growing microbes in order to observe the difference in CO2 solubility. Factors of temperature and pressure were also studied. The determination of CO2 concentration in the solution was measured by gas analyzer. The result obtained from optimization revealed a maximum CO2 concentration of 19.029 mol/L in the culture media with MEA, at a pressure of 136.728 kPa, operating at 20.483 °C.
Keywords
Carbon Dioxide, culture media, microorganism, Optimization
Subject
Suggested Citation
Adnan AI, Ong MY, Nomanbhay S, Show PL. Determination of Dissolved CO2 Concentration in Culture Media: Evaluation of pH Value and Mathematical Data. (2021). LAPSE:2021.0375v1
Author Affiliations
Adnan AI: Institute of Sustainable Energy, Universiti Tenaga Nasional, Kajang 43000, Selangor, Malaysia
Ong MY: Institute of Sustainable Energy, Universiti Tenaga Nasional, Kajang 43000, Selangor, Malaysia
Nomanbhay S: Institute of Sustainable Energy, Universiti Tenaga Nasional, Kajang 43000, Selangor, Malaysia
Show PL: Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor, Malaysia [ORCID]
Journal Name
Processes
Volume
8
Issue
11
Article Number
E1373
Year
2020
Publication Date
2020-10-29
Published Version
ISSN
2227-9717
Version Comments
Original Submission
Other Meta
PII: pr8111373, Publication Type: Journal Article
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LAPSE:2021.0375v1
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doi:10.3390/pr8111373
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May 17, 2021
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CC BY 4.0
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[v1] (Original Submission)
May 17, 2021
 
Verified by curator on
May 17, 2021
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http://psecommunity.org/LAPSE:2021.0375v1
 
Original Submitter
Calvin Tsay
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