LAPSE:2021.0293
Published Article
LAPSE:2021.0293
Real-Time Nanoplasmonic Sensor for IgG Monitoring in Bioproduction
Thuy Tran, Olof Eskilson, Florian Mayer, Robert Gustavsson, Robert Selegård, Ingemar Lundström, Carl-Fredrik Mandenius, Erik Martinsson, Daniel Aili
April 29, 2021
Real-time monitoring of product titers during process development and production of biotherapeutics facilitate implementation of quality-by-design principles and enable rapid bioprocess decision and optimization of the production process. Conventional analytical methods are generally performed offline/at-line and, therefore, are not capable of generating real-time data. In this study, a novel fiber optical nanoplasmonic sensor technology was explored for rapid IgG titer measurements. The sensor combines localized surface plasmon resonance transduction and robust single use Protein A-modified sensor chips, housed in a flexible flow cell, for specific IgG detection. The sensor requires small sample volumes (1−150 µL) and shows a reproducibility and sensitivity comparable to Protein G high performance liquid chromatography-ultraviolet (HPLC-UV). The dynamic range of the sensor system can be tuned by varying the sample volume, which enables quantification of IgG samples ranging from 0.0015 to 10 mg/mL, without need for sample dilution. The sensor shows limited interference from the sample matrix and negligible unspecific protein binding. IgG titers can be rapidly determined in samples from filtered unpurified Chinese hamster ovary (CHO) cell cultures and show good correlation with enzyme-linked immunosorbent assay (ELISA).
Keywords
bioprocess, IgG titer, nanoplasmonic, on-line, PAT, real-time
Suggested Citation
Tran T, Eskilson O, Mayer F, Gustavsson R, Selegård R, Lundström I, Mandenius CF, Martinsson E, Aili D. Real-Time Nanoplasmonic Sensor for IgG Monitoring in Bioproduction. (2021). LAPSE:2021.0293
Author Affiliations
Tran T: Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden
Eskilson O: Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden
Mayer F: Laboratory of Biotechnology, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden [ORCID]
Gustavsson R: Laboratory of Biotechnology, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden
Selegård R: Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden [ORCID]
Lundström I: Sensor and Actuator Systems, Department of Physics, Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden
Mandenius CF: Laboratory of Biotechnology, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden
Martinsson E: ArgusEye AB, Spannmålsgatan 55, 583 36 Linköping, Sweden
Aili D: Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden [ORCID]
Journal Name
Processes
Volume
8
Issue
10
Article Number
E1302
Year
2020
Publication Date
2020-10-16
Published Version
ISSN
2227-9717
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Original Submission
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PII: pr8101302, Publication Type: Journal Article
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LAPSE:2021.0293
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doi:10.3390/pr8101302
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Apr 29, 2021
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CC BY 4.0
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Apr 29, 2021
 
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Calvin Tsay
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