LAPSE:2021.0061
Published Article
LAPSE:2021.0061
Thermostable α-Glucan Phosphorylase-Catalyzed Enzymatic Copolymerization to Produce Partially 2-Deoxygenated Amyloses
Jun-ichi Kadokawa, Shota Nakamura, Kazuya Yamamoto
February 22, 2021
α-Glucan phosphorylase catalyzes the enzymatic polymerization of α-d-glucose 1-phosphate (Glc-1-P) monomers from a maltooligosaccharide primer to produce α(1→4)-glucan—i.e., amylose. In this study, by exploiting the weak specificity for the substrate recognition of a thermostable α-glucan phosphorylase (from Aquifex aeolicus VF5), we investigated the enzymatic copolymerization of 2-deoxy-α-d-glucose 1-phosphate (dGlc-1-P), which was produced in situ from d-glucal, with Glc-1-P to obtain non-natural heteropolysaccharides composed of α(1→4)-linked dGlc/Glc units—i.e., partially 2-deoxygenated amylose. The reactions were carried out at different monomer feed ratios using a maltotriose primer at 40 °C for 24 h. The products were precipitated from the reaction medium, isolated by centrifugation, and subjected to 1H NMR spectroscopic and powder X-ray diffraction measurements to evaluate their chemical and crystalline structures, respectively. Owing to its amorphous nature, the partially 2-deoxygenated amylose with adapted unit ratios formed a film when subjected to a casting method.
Keywords
2-deoxyamylose, d-glucal, enzymatic copolymerization, heteropolysaccharide, α-glucan phosphorylase
Suggested Citation
Kadokawa JI, Nakamura S, Yamamoto K. Thermostable α-Glucan Phosphorylase-Catalyzed Enzymatic Copolymerization to Produce Partially 2-Deoxygenated Amyloses. (2021). LAPSE:2021.0061
Author Affiliations
Kadokawa JI: Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan [ORCID]
Nakamura S: Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan
Yamamoto K: Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan
Journal Name
Processes
Volume
8
Issue
9
Article Number
E1070
Year
2020
Publication Date
2020-09-01
Published Version
ISSN
2227-9717
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Original Submission
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PII: pr8091070, Publication Type: Journal Article
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LAPSE:2021.0061
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doi:10.3390/pr8091070
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Feb 22, 2021
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Feb 22, 2021
 
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Feb 22, 2021
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Calvin Tsay
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