LAPSE:2020.1155
Published Article
LAPSE:2020.1155
A Novel Cysteine-Functionalized MxOy Material as Support for Laccase Immobilization and a Potential Application in Decolorization of Alizarin Red S
Agnieszka Kołodziejczak-Radzimska, Teofil Jesionowski
November 11, 2020
Immobilization process improves the enzyme properties, like stability, activity, selectivity or specificity. In the study, a novel cysteine-functionalized MxOy (ZrO2, SiO2) material was used as a support for the immobilization of laccase from Trametes versicolor. The proposed matrix was prepared using a simple sol-gel method. The cysteine was introduced during the synthesis of a sample. Additionally, the obtained supports were modified with glutaraldehyde. The basic properties of the prepared cysteine functionalized ZrO2 and SiO2 were determined using spectroscopic, thermal, porous, electrostatic and elemental analysis. Furthermore, the obtained biocatalytic systems were used as catalysts in the oxidation of sulfonic acid. Catalytic and kinetic parameters were determined based on the proposed model reaction. Next, laccase immobilized on ZrO2- and SiO2-based materials were, for the first time, utilized in the decolorization of Alizarin Red S. In that process, the influence of duration, pH and temperature on the efficiency of decolorization was evaluated. The results show that the proposed biocatalytic systems offer good specific activity (ca. 19 U/mg) and activity retention (ca. 77%). Importantly, they can be successfully used in the decolorization of Alizarin Red S with high efficiency (above 95%).
Keywords
Catalysis, decolorization, kinetic, l-cysteine, laccase, SiO2, ZrO2
Subject
Suggested Citation
Kołodziejczak-Radzimska A, Jesionowski T. A Novel Cysteine-Functionalized MxOy Material as Support for Laccase Immobilization and a Potential Application in Decolorization of Alizarin Red S. (2020). LAPSE:2020.1155
Author Affiliations
Kołodziejczak-Radzimska A: Faculty of Chemical Technology, Institute of Technology and Chemical Engineering, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland
Jesionowski T: Faculty of Chemical Technology, Institute of Technology and Chemical Engineering, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland [ORCID]
Journal Name
Processes
Volume
8
Issue
8
Article Number
E885
Year
2020
Publication Date
2020-07-23
Published Version
ISSN
2227-9717
Version Comments
Original Submission
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PII: pr8080885, Publication Type: Journal Article
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LAPSE:2020.1155
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doi:10.3390/pr8080885
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Nov 11, 2020
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CC BY 4.0
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Nov 11, 2020
 
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Nov 11, 2020
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Original Submitter
Calvin Tsay
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