LAPSE:2020.0932
Published Article
LAPSE:2020.0932
Dynamic Metabolic Analysis of Cupriavidus necator DSM545 Producing Poly(3-hydroxybutyric acid) from Glycerol
Chenhao Sun, Cristina Pérez-Rivero, Colin Webb, Constantinos Theodoropoulos
August 29, 2020
Cupriavidus necator DSM 545 can utilise glycerol to synthesise poly(3-hydroxybutyric acid) under unbalanced growth conditions, i.e., nitrogen limitation. To improve poly(3-hydroxybutyric acid) (PHB) batch production by C. necator through model-guided bioprocessing or genetic engineering, insights into the dynamic effect of the fermentation conditions on cell metabolism are crucial. In this work, we have used dynamic flux balance analysis (DFBA), a constrained-based stoichiometric modelling approach, to study the metabolic change associated with PHB synthesis during batch cultivation. The model employs the ‘minimisation of all fluxes’ as cellular objectives and measured extracellular fluxes as additional constraints. The mass balance constraints are further adjusted based on thermodynamic considerations. The resultant flux distribution profiles characterise the evolution of metabolic states due to adaptation to dynamic extracellular conditions and provide further insights towards improvements that can be implemented to enhance PHB productivity.
Keywords
bioprocess, constraint-based stoichiometric modelling, data-driven approach, flux balance analysis, metabolic modelling, poly(3-hydroxybutyric acid), pseudo-steady state, thermodynamics
Subject
Suggested Citation
Sun C, Pérez-Rivero C, Webb C, Theodoropoulos C. Dynamic Metabolic Analysis of Cupriavidus necator DSM545 Producing Poly(3-hydroxybutyric acid) from Glycerol. (2020). LAPSE:2020.0932
Author Affiliations
Sun C: Future Biomanufacturing Research Hub, Manchester Institute of Biotechnology, Department of Chemistry, University of Manchester, Manchester M13 9PL, UK; Department of Chemical Engineering and Analytical Science, The University of Manchester, Manchester M13
Pérez-Rivero C: Department of Chemical Engineering and Analytical Science, The University of Manchester, Manchester M13 9PL, UK
Webb C: Department of Chemical Engineering and Analytical Science, The University of Manchester, Manchester M13 9PL, UK [ORCID]
Theodoropoulos C: Future Biomanufacturing Research Hub, Manchester Institute of Biotechnology, Department of Chemistry, University of Manchester, Manchester M13 9PL, UK; Department of Chemical Engineering and Analytical Science, The University of Manchester, Manchester M13
Journal Name
Processes
Volume
8
Issue
6
Article Number
E657
Year
2020
Publication Date
2020-06-01
Published Version
ISSN
2227-9717
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Original Submission
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PII: pr8060657, Publication Type: Journal Article
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LAPSE:2020.0932
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doi:10.3390/pr8060657
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Aug 29, 2020
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CC BY 4.0
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Aug 29, 2020
 
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Original Submitter
Calvin Tsay
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