LAPSE:2020.0920
Published Article
LAPSE:2020.0920
A Novel Approach in Crude Enzyme Laccase Production and Application in Emerging Contaminant Bioremediation
August 5, 2020
Laccase enzyme from white-rot fungi is a potential biocatalyst for the oxidation of emerging contaminants (ECs), such as pesticides, pharmaceuticals and steroid hormones. This study aims to develop a three-step platform to treat ECs: (i) enzyme production, (ii) enzyme concentration and (iii) enzyme application. In the first step, solid culture and liquid culture were compared. The solid culture produced significantly more laccase than the liquid culture (447 vs. 74 µM/min after eight days), demonstrating that white rot fungi thrived on a solid medium. In the second step, the enzyme was concentrated 6.6 times using an ultrafiltration (UF) process, resulting in laccase activity of 2980 µM/min. No enzymatic loss due to filtration and membrane adsorption was observed, suggesting the feasibility of the UF membrane for enzyme concentration. In the third step, concentrated crude enzyme was applied in an enzymatic membrane reactor (EMR) to remove a diverse set of ECs (31 compounds in six groups). The EMR effectively removed of steroid hormones, phytoestrogen, ultraviolet (UV) filters and industrial chemical (above 90%). However, it had low removal of pesticides and pharmaceuticals.
Record ID
Keywords
crude enzyme laccase, emerging contaminants, enzymatic degradation, enzymatic membrane reactor, membrane filtration, white-rot fungi
Subject
Suggested Citation
Nguyen LN, Vu MT, Johir MAH, Pathak N, Zdarta J, Jesionowski T, Semblante GU, Hai FI, Khanh Dieu Nguyen H, Nghiem LD. A Novel Approach in Crude Enzyme Laccase Production and Application in Emerging Contaminant Bioremediation. (2020). LAPSE:2020.0920
Author Affiliations
Nguyen LN: Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2220, Australia [ORCID]
Vu MT: Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2220, Australia [ORCID]
Johir MAH: Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2220, Australia
Pathak N: Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2220, Australia
Zdarta J: Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60965 Poznan, Poland [ORCID]
Jesionowski T: Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60965 Poznan, Poland [ORCID]
Semblante GU: Technical Services, Western Sydney University, Kingswood, NSW 2747, Australia
Hai FI: Strategic Water Infrastructure Lab, School of Civil, Mining and Environmental Engineering, University of Wollongong, Wollongong, NSW 2522, Australia
Khanh Dieu Nguyen H: School of Chemical Engineering, Ha Noi University of Science and Technology, Ha Noi 100000, Vietnam [ORCID]
Nghiem LD: Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2220, Australia
Vu MT: Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2220, Australia [ORCID]
Johir MAH: Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2220, Australia
Pathak N: Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2220, Australia
Zdarta J: Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60965 Poznan, Poland [ORCID]
Jesionowski T: Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60965 Poznan, Poland [ORCID]
Semblante GU: Technical Services, Western Sydney University, Kingswood, NSW 2747, Australia
Hai FI: Strategic Water Infrastructure Lab, School of Civil, Mining and Environmental Engineering, University of Wollongong, Wollongong, NSW 2522, Australia
Khanh Dieu Nguyen H: School of Chemical Engineering, Ha Noi University of Science and Technology, Ha Noi 100000, Vietnam [ORCID]
Nghiem LD: Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2220, Australia
Journal Name
Processes
Volume
8
Issue
6
Article Number
E648
Year
2020
Publication Date
2020-05-29
Published Version
ISSN
2227-9717
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Original Submission
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PII: pr8060648, Publication Type: Journal Article
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Published Article
LAPSE:2020.0920
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External Link
doi:10.3390/pr8060648
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Version History
[v1] (Original Submission)
Aug 5, 2020
Verified by curator on
Aug 5, 2020
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v1
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https://psecommunity.org/LAPSE:2020.0920
Original Submitter
Calvin Tsay
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