LAPSE:2020.0393
Published Article
LAPSE:2020.0393
Application of a Liquid Biphasic Flotation (LBF) System for Protein Extraction from Persiscaria Tenulla Leaf
April 14, 2020
Persiscaria tenulla, commonly known as Polygonum, is a plant belonging to the family Polygonaceae, which originated from and is widely found in Southeast Asia countries, such as Indonesia, Malaysia, Thailand, and Vietnam. The leaf of the plant is believed to have active ingredients that are responsible for therapeutic effects. In order to take full advantage of a natural medicinal plant for the application in the pharmaceutical and food industries, extraction and separation techniques are essential. In this study, an emerging and rapid extraction approach known as liquid biphasic flotation (LBF) is proposed for the extraction of protein from Persiscaria tenulla leaves. The scope of this study is to establish an efficient, environmentally friendly, and cost-effective technology for the extraction of protein from therapeutic leaves. Based on the ideal conditions of the small LBF system, a 98.36% protein recovery yield and a 79.12% separation efficiency were achieved. The upscaling study of this system exhibited the reliability of this technology for large-scale applications with a protein recovery yield of 99.44% and a separation efficiency of 93.28%. This technology demonstrated a simple approach with an effective protein recovery yield and separation that can be applied for the extraction of bioactive compounds from various medicinal-value plants.
Record ID
Keywords
Extraction, leaf, liquid biphasic flotation, polygonum, protein
Subject
Suggested Citation
Saw HS, Sankaran R, Khoo KS, Chew KW, Phong WN, Tang MS, Lim SS, Mohd Zaid HF, Naushad M, Show PL. Application of a Liquid Biphasic Flotation (LBF) System for Protein Extraction from Persiscaria Tenulla Leaf. (2020). LAPSE:2020.0393
Author Affiliations
Saw HS: School of Biosciences, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500 Selangor Darul Ehsan, Malaysia
Sankaran R: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; Research and Training Department, Graduate School, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500 Selangor Darul Ehsan, Malaysia [ORCID]
Khoo KS: Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500 Selangor Darul Ehsan, Malaysia [ORCID]
Chew KW: School of Mathematical Sciences, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500 Selangor Darul Ehsan, Malaysia
Phong WN: School of Molecular and Life Sciences, Curtin University, Bentley, GPO Box U1987, Perth, WA 6845, Australia
Tang MS: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
Lim SS: Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500 Selangor Darul Ehsan, Malaysia
Mohd Zaid HF: Fundamental and Applied Sciences Department, Centre of Innovative Nanostructures and Nanodevices, Institute of Autonomous Systems, Universiti Teknologi PETRONAS, 32610 Bandar Seri Iskandar, Malaysia
Naushad M: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia [ORCID]
Show PL: Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500 Selangor Darul Ehsan, Malaysia
Sankaran R: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; Research and Training Department, Graduate School, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500 Selangor Darul Ehsan, Malaysia [ORCID]
Khoo KS: Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500 Selangor Darul Ehsan, Malaysia [ORCID]
Chew KW: School of Mathematical Sciences, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500 Selangor Darul Ehsan, Malaysia
Phong WN: School of Molecular and Life Sciences, Curtin University, Bentley, GPO Box U1987, Perth, WA 6845, Australia
Tang MS: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
Lim SS: Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500 Selangor Darul Ehsan, Malaysia
Mohd Zaid HF: Fundamental and Applied Sciences Department, Centre of Innovative Nanostructures and Nanodevices, Institute of Autonomous Systems, Universiti Teknologi PETRONAS, 32610 Bandar Seri Iskandar, Malaysia
Naushad M: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia [ORCID]
Show PL: Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500 Selangor Darul Ehsan, Malaysia
Journal Name
Processes
Volume
8
Issue
2
Article Number
E247
Year
2020
Publication Date
2020-02-21
Published Version
ISSN
2227-9717
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Original Submission
Other Meta
PII: pr8020247, Publication Type: Journal Article
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LAPSE:2020.0393
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External Link
doi:10.3390/pr8020247
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Version History
[v1] (Original Submission)
Apr 14, 2020
Verified by curator on
Apr 14, 2020
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v1
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https://psecommunity.org/LAPSE:2020.0393
Original Submitter
Calvin Tsay
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