LAPSE:2020.0296
Published Article
LAPSE:2020.0296
Modification of the Bacterial Cell Wall—Is the Bioavailability Important in Creosote Biodegradation?
March 12, 2020
Creosote oil, widely used as a wood preservative, is a complex mixture of different polycyclic aromatic compounds. The soil contamination result in the presence of a specific microcosm. The presented study focuses on the most active strains involved in bioremediation of long-term creosote-contaminated soil. In three soil samples from different boreholes, two Sphingomonas maltophilia (S. maltophilia) and one Paenibacillus ulginis (P. ulginis) strain were isolated. The conducted experiments showed the differences and similarities between the bacteria strains capable of degrading creosote from the same contaminated area. Both S. maltophilia strains exhibit higher biodegradation efficiency (over 50% after 28 days) and greater increase in glutathione S-transferase activity than P. ulginis ODW 5.9. However, S. maltophilia ODW 3.7 and P. ulginis ODW 5.9 were different from the third of the tested strains. The growth of the former two on creosote resulted in an increase in cell adhesion to Congo red and in the total membrane permeability. Nevertheless, all three strains have shown a decrease in the permeability of the inner cell membrane. That suggests the complex relationship between the cell surface modifications and bioavailability of the creosote to microorganisms. The conducted research allowed us to broaden the current knowledge about the creosote bioremediation and the properties of microorganisms involved in the process.
Keywords
bacteria, bioavailability, biodegradation, creosote, PAHs
Subject
Suggested Citation
Smułek W, Pacholak A, Kaczorek E. Modification of the Bacterial Cell Wall—Is the Bioavailability Important in Creosote Biodegradation?. (2020). LAPSE:2020.0296
Author Affiliations
Smułek W: Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60-695 Poznan, Poland [ORCID]
Pacholak A: Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60-695 Poznan, Poland [ORCID]
Kaczorek E: Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60-695 Poznan, Poland [ORCID]
Journal Name
Processes
Volume
8
Issue
2
Article Number
E147
Year
2020
Publication Date
2020-01-23
Published Version
ISSN
2227-9717
Version Comments
Original Submission
Other Meta
PII: pr8020147, Publication Type: Journal Article
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LAPSE:2020.0296
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doi:10.3390/pr8020147
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Mar 12, 2020
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CC BY 4.0
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Mar 12, 2020
 
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Mar 12, 2020
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https://psecommunity.org/LAPSE:2020.0296
 
Original Submitter
Calvin Tsay
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