LAPSE:2020.0172
Published Article
LAPSE:2020.0172
Cross-Linking of Fibrex Gel by Fungal Laccase: Gel Rheological and Structural Characteristics
February 3, 2020
Sugar beet fibre (fibrex) is an abundant side-stream from the sugar refining industry. A self-produced laccase from Funalia trogii (LccFtr) (0.05 U/µg FA) successfully cross-linked fibrex to an edible gel. Dynamic oscillation measurements of the 10% fibrex gels showed a storage modulus of 5.52 kPa and loss factors ≤ 0.36 in the range from 20 to 80 Hz. Comparing storage stability of sweetened 10% fibrex gels with sweetened commercial 6% gelatin gels (10% and 30% d-sucrose) indicated a constant storage modulus and loss factors ≤ 0.7 during four weeks of storage in fibrex gels. Loss factors of sweetened gelatin gels were ≤0.2, and their storage modulus decreased from 9 to 7 kPa after adding d-sucrose and remained steady for four weeks of storage. Fibrex gel characteristics, including high water holding capacity, swelling ratio in saliva, and heat resistance are attributed to a covalently cross-linked network. Vanillin, as a mediator, and citrus pectin did not enhance covalent cross-links and elastic properties of the fibrex gels. Thus, laccase as an oxidative agent provided gels with a solid and stable texture. Fibrex gels may find uses in pharmaceutical and other industrial applications, which require a heat-resistant gel that forms easily at room temperature. They also represent an ethical alternative for manufacturing vegan, halal, and kosher food.
Keywords
cross-linking, fibrex gel, laccase, rheology, viscoelastic properties
Subject
Suggested Citation
Khalighi S, Berger RG, Ersoy F. Cross-Linking of Fibrex Gel by Fungal Laccase: Gel Rheological and Structural Characteristics. (2020). LAPSE:2020.0172
Author Affiliations
Khalighi S: Institute of food chemistry, Leibniz University Hannover, Callinstr. 5, 30167 Hannover, Germany [ORCID]
Berger RG: Institute of food chemistry, Leibniz University Hannover, Callinstr. 5, 30167 Hannover, Germany [ORCID]
Ersoy F: Institute of food chemistry, Leibniz University Hannover, Callinstr. 5, 30167 Hannover, Germany [ORCID]
Journal Name
Processes
Volume
8
Issue
1
Article Number
E16
Year
2019
Publication Date
2019-12-20
Published Version
ISSN
2227-9717
Version Comments
Original Submission
Other Meta
PII: pr8010016, Publication Type: Journal Article
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LAPSE:2020.0172
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doi:10.3390/pr8010016
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Feb 3, 2020
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CC BY 4.0
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Feb 3, 2020
 
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Feb 3, 2020
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Original Submitter
Calvin Tsay
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