Recovery of Protein from Dairy Milk Waste Product Using Alcohol-Salt Liquid Biphasic Flotation

Pei En Tham; Yan Jer Ng; Revathy Sankaran; Kuan Shiong Khoo; Kit Wayne Chew; Yee Jiun Yap; Masnindah Malahubban; Fitri Abdul Aziz Zakry; Pau Loke Show

LAPSE

Living Archive for Process Systems Engineering

LAPSE:2020.0014

Published Article

LAPSE:2020.0014

Recovery of Protein from Dairy Milk Waste Product Using Alcohol-Salt Liquid Biphasic Flotation

Pei En Tham, Yan Jer Ng, Revathy Sankaran, Kuan Shiong Khoo, Kit Wayne Chew, Yee Jiun Yap, Masnindah Malahubban, Fitri Abdul Aziz Zakry, Pau Loke Show

January 2, 2020

Expired dairy products are often disposed of due to the potential health hazard they pose to living organisms. Lack of methods to recover valuable components from them are also a reason for manufactures to dispose of the expired dairy products. Milk encompasses several different components with their own functional properties that can be applied in production of food and non-food technical products. This study aims to investigate the novel approach of using liquid biphasic flotation (LBF) method for protein extraction from expired milk products and obtaining the optimal operating conditions for protein extraction. The optimized conditions were found at 80% concentration ethanol as top phase, 150 g/L dipotassium hydrogen phosphate along with 10% (w/v) milk as bottom phase, and a flotation time of 7.5 min. The protein recovery yield and separation efficiency after optimization were 94.97% and 86.289%, respectively. The experiment has been scaled up by 40 times to ensure it can be commercialized, and the protein recovery yield and separation efficiency were found to be 78.92% and 85.62%, respectively. This novel approach gives a chance for expired milk products to be changed from waste to raw materials which is beneficial for the environment and the economy.

Record ID

LAPSE:2020.0014

Keywords

dairy waste, liquid biphasic flotation, milk, protein, recovery

Subject

Process Design

Suggested Citation

Tham PE, Ng YJ, Sankaran R, Khoo KS, Chew KW, Yap YJ, Malahubban M, Aziz Zakry FA, Show PL. Recovery of Protein from Dairy Milk Waste Product Using Alcohol-Salt Liquid Biphasic Flotation. (2020). LAPSE:2020.0014

Author Affiliations

Tham PE: Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia
Ng YJ: Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia
Sankaran R: Institute of Biological Sciences, Faculty of Science, University of Malaya, Jalan Universiti, Kuala Lumpur 50603, Malaysia
Khoo KS: Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia
Chew KW: School of Mathematical Sciences, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia [ORCID]
Yap YJ: School of Mathematical Sciences, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia
Malahubban M: Faculty of Agriculture and Food Sciences, Universiti Putra Malaysia Sarawak Campus, Bintulu 97008, Sarawak, Malaysia
Aziz Zakry FA: Faculty of Agriculture and Food Sciences, Universiti Putra Malaysia Sarawak Campus, Bintulu 97008, Sarawak, Malaysia
Show PL: Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia [ORCID]

Journal Name

Processes

Volume

7

Issue

12

Article Number

E875

Year

2019

Publication Date

2019-11-21