LAPSE:2019.0953
Published Article
LAPSE:2019.0953
Lipid Isolation Process and Study on Some Molecular Species of Polar Lipid Isolated from Seed of Madhuca ellitica
Doan Lan Phuong, Tran Quoc Toan, Ly P. T. Dang, Andrey B. Imbs, Pham Quoc Long, Tran Dinh Thang, Bertrand Matthaeus, Long Giang Bach, Le Minh Bui
August 14, 2019
This study attempted the lipid extraction process from the seeds of Madhuca ellitica, a lipid-rich plant, and conducted a lipidomic analysis on molecular species of the obtained product. Total lipids of the crude seeds were found to contain 11.2% of polar lipids. The major fatty acids (FAs) of the polar lipids were palmitic (16:0), stearic (18:0), oleic (18:1n-9), and linoleic (18:2n-6) acids, which amounted to 28.5, 12.5, 44.8, and 13.2% of total FAs, respectively. The content and chemical structures of individual molecular species of phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid (PA), and sulfoquinovosyldiacylglycerol (SQDG) were determined by HPLC with a tandem high-resolution mass spectrometry (HRMS). The major molecular species were 18:1/18:2 PE, 16:0/18:1 PC, 18:1/18:2 PC, 16:0/18:2 PG, 16:0/18:1 PG, 16:1/18:1 PI, 16:0/18:1 PI, 18:0/18:2 PI, 16:0/18:1 PA, 18:1/18:2 PA, 16:0/18:1 SQDG, and 18:0/18:1 SQDG. The application of a tandem HRMS allows us to determine the content of each isomer in pairs of the monoisotopic molecular species, for example, 18:0/18:2 and 18:1/18:1. The evaluation of the seed polar lipid profile will be helpful for developing the potential of this tree for nutritive and industrial uses.
Keywords
lipid molecular species, Madhuca ellitica, phospholipids, seeds, sulfoquinovosyldiacylglycerol, tandem high-resolution mass spectrometry
Subject
Suggested Citation
Phuong DL, Toan TQ, Dang LPT, Imbs AB, Long PQ, Thang TD, Matthaeus B, Bach LG, Bui LM. Lipid Isolation Process and Study on Some Molecular Species of Polar Lipid Isolated from Seed of Madhuca ellitica. (2019). LAPSE:2019.0953
Author Affiliations
Phuong DL: Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet road, Caugiay district, Hanoi 10000, Vietnam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology (VAST), 18
Toan TQ: Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet road, Caugiay district, Hanoi 10000, Vietnam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology (VAST), 18 [ORCID]
Dang LPT: Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet road, Caugiay district, Hanoi 10000, Vietnam
Imbs AB: A.V. Zhirmunsky Institute of Marine Biology, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok 690041, Russia
Long PQ: Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet road, Caugiay district, Hanoi 10000, Vietnam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology (VAST), 18
Thang TD: School of Chemistry, Biology and Environment, Vinh University, Vinh City 46000, Vietnam
Matthaeus B: Working Group of Lipid Research, Department of Safety and Quality of Cereals Max, Federal Research Institute for Nutrition and Food, Rubner-Institut, Schutzenberg 12, DE-32756 Detmold, Germany [ORCID]
Bach LG: NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City 70000, Vietnam; Center of Excellence for Biochemistry and Natural Products, Nguyen Tat Thanh University, Ho Chi Minh City 70000, Vietnam
Bui LM: NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City 70000, Vietnam; Center of Excellence for Biochemistry and Natural Products, Nguyen Tat Thanh University, Ho Chi Minh City 70000, Vietnam [ORCID]
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Journal Name
Processes
Volume
7
Issue
6
Article Number
E375
Year
2019
Publication Date
2019-06-17
Published Version
ISSN
2227-9717
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Original Submission
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PII: pr7060375, Publication Type: Journal Article
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LAPSE:2019.0953
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doi:10.3390/pr7060375
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Aug 14, 2019
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Calvin Tsay
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