LAPSE:2019.0841
Published Article
LAPSE:2019.0841
Optimization of Ultrasound-Assisted Extraction on Antioxidative Activity of Malus toringoides Using Response Surface Methodology
July 30, 2019
Ultrasonic-assisted extraction (UAE) was optimized using response surface methodology (RSM) to maintain the cyto-protective activity of M.toringoides against oxidative stress. The optimal conditions for UAE were a 58 mL/g liquid-solid ratio, a 38 °C extraction temperature, an 85% solvent concentration, and a 19-min extraction time, which resulted in a protection rate of 54.57% against hydrogen peroxide-induced oxidative stress in human umbilical vein endothelial cells (HUVECs). These results were comparable to the predicted value of 53.75%. The extracts showed excellent antioxidant activity, and phlorizin was detected in the dried leaves of Malus.toringoides. The highest yield of phlorizin (101.239 mg/g) was also obtained using these conditions. Taken together, these results showed that the method successfully integrated RSM and partial least squares regression methods to optimize M.toringoides extraction to yield the highest cyto-protective activity and effectively increase the yield of phlorizin from M.toringoides.
Record ID
Keywords
human umbilical vein endothelial cells, M.toringoides, protection rate, response surface methodology, ultrasonic-assisted extraction
Subject
Suggested Citation
Ye Q, Guo L, Liu H, Liu Y, Zhang C, Peng C, Liu Z, Huang S, Li B. Optimization of Ultrasound-Assisted Extraction on Antioxidative Activity of Malus toringoides Using Response Surface Methodology. (2019). LAPSE:2019.0841
Author Affiliations
Ye Q: The College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; The Breeding Base of State Key Laboratory of Resources Systems Research and Development Utilization of Chinese Herbal Medicines Constructioned by The Minis
Guo L: The College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; The Breeding Base of State Key Laboratory of Resources Systems Research and Development Utilization of Chinese Herbal Medicines Constructioned by The Minis
Liu H: The College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; The Breeding Base of State Key Laboratory of Resources Systems Research and Development Utilization of Chinese Herbal Medicines Constructioned by The Minis
Liu Y: The College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; The Breeding Base of State Key Laboratory of Resources Systems Research and Development Utilization of Chinese Herbal Medicines Constructioned by The Minis
Zhang C: The College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; The Breeding Base of State Key Laboratory of Resources Systems Research and Development Utilization of Chinese Herbal Medicines Constructioned by The Minis
Peng C: The College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; The Breeding Base of State Key Laboratory of Resources Systems Research and Development Utilization of Chinese Herbal Medicines Constructioned by The Minis
Liu Z: Department of Pharmacy, Qingdao University of Science and Technology, Qingdao 266000, China
Huang S: Department of Pharmacy, Qingdao University of Science and Technology, Qingdao 266000, China
Li B: Department of Pharmacy, Qingdao University of Science and Technology, Qingdao 266000, China [ORCID]
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Guo L: The College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; The Breeding Base of State Key Laboratory of Resources Systems Research and Development Utilization of Chinese Herbal Medicines Constructioned by The Minis
Liu H: The College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; The Breeding Base of State Key Laboratory of Resources Systems Research and Development Utilization of Chinese Herbal Medicines Constructioned by The Minis
Liu Y: The College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; The Breeding Base of State Key Laboratory of Resources Systems Research and Development Utilization of Chinese Herbal Medicines Constructioned by The Minis
Zhang C: The College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; The Breeding Base of State Key Laboratory of Resources Systems Research and Development Utilization of Chinese Herbal Medicines Constructioned by The Minis
Peng C: The College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; The Breeding Base of State Key Laboratory of Resources Systems Research and Development Utilization of Chinese Herbal Medicines Constructioned by The Minis
Liu Z: Department of Pharmacy, Qingdao University of Science and Technology, Qingdao 266000, China
Huang S: Department of Pharmacy, Qingdao University of Science and Technology, Qingdao 266000, China
Li B: Department of Pharmacy, Qingdao University of Science and Technology, Qingdao 266000, China [ORCID]
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Journal Name
Processes
Volume
7
Issue
5
Article Number
E270
Year
2019
Publication Date
2019-05-08
Published Version
ISSN
2227-9717
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Original Submission
Other Meta
PII: pr7050270, Publication Type: Journal Article
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Published Article
LAPSE:2019.0841
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External Link
doi:10.3390/pr7050270
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Version History
[v1] (Original Submission)
Jul 30, 2019
Verified by curator on
Jul 30, 2019
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v1
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https://psecommunity.org/LAPSE:2019.0841
Original Submitter
Calvin Tsay
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