LAPSE:2019.0581
Published Article
LAPSE:2019.0581
Purification of Flavonoids from Mulberry Leaves via High-Speed Counter-Current Chromatography
Pian Zhang, Kang-Ling Zhu, Jun Zhang, Yan Li, Heng Zhang, Yan Wang
June 10, 2019
In order to obtain high-purity flavonoid products, the extracts from mulberry leaves were separated and purified via high-speed counter-current chromatography (HSCCC). Moreover, the product was detected via high-performance liquid chromatography (HPLC). The characteristic absorption wavelength of the rutin standard for HSCCC detection and HPLC analysis at 257 nm was tested by ultraviolet scanning analysis. The effect of solvent systems and mobile phase flow rate on the separation efficiency were then researched. Finally, the solvent system of V(ethyl acetate):V(n-butanol):V(water) = 4:1:5 was selected as the operating system for HSCCC. This work theoretically analyzed the impact of the molecular structure and polarity of flavonoids on the choice of solvent systems. The results showed that the mobile phase flow rate had a great influence on the separation efficiency. Furthermore, the separation efficiency increased as the mobile phase flow rate decreased. When the mobile phase flow rate was 5 mL/min, the peak time for flavonoids was 140 min, the retention of the stationary phase was 56.4%, and the purity of the product reached 93.8%. The results of this study greatly improved the purity of flavonoids in mulberry leaf and provided a strong support for the separation and purification of mulberry leaf extract.
Keywords
flavonoids, high-performance liquid chromatography, high-speed counter-current chromatography, mulberry leaf, separation
Subject
Suggested Citation
Zhang P, Zhu KL, Zhang J, Li Y, Zhang H, Wang Y. Purification of Flavonoids from Mulberry Leaves via High-Speed Counter-Current Chromatography. (2019). LAPSE:2019.0581
Author Affiliations
Zhang P: Institution of chemical engineering, Huazhong University of Science and Technology, Wuhan 430074, China [ORCID]
Zhu KL: Institution of chemical engineering, Huazhong University of Science and Technology, Wuhan 430074, China
Zhang J: Institution of chemical engineering, Huazhong University of Science and Technology, Wuhan 430074, China
Li Y: Institution of chemical engineering, Huazhong University of Science and Technology, Wuhan 430074, China
Zhang H: Institution of chemical engineering, Huazhong University of Science and Technology, Wuhan 430074, China
Wang Y: Institution of chemical engineering, Huazhong University of Science and Technology, Wuhan 430074, China
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Journal Name
Processes
Volume
7
Issue
2
Article Number
E91
Year
2019
Publication Date
2019-02-13
Published Version
ISSN
2227-9717
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PII: pr7020091, Publication Type: Journal Article
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LAPSE:2019.0581
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doi:10.3390/pr7020091
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Jun 10, 2019
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