Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology

Abdi Hanra Sebayang; Masjuki Haji Hassan; Hwai Chyuan Ong; Surya Dharma; Arridina Susan Silitonga; Fitranto Kusumo; Teuku Meurah Indra Mahlia; Aditiya Harjon Bahar

LAPSE

Living Archive for Process Systems Engineering

LAPSE:2019.0441

Published Article

LAPSE:2019.0441

Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology

Abdi Hanra Sebayang, Masjuki Haji Hassan, Hwai Chyuan Ong, Surya Dharma, Arridina Susan Silitonga, Fitranto Kusumo, Teuku Meurah Indra Mahlia, Aditiya Harjon Bahar

March 26, 2019

Bioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was performed with selected hydrolysis parameters, including substrate loading, stroke speed, α-amylase concentration and amyloglucosidase concentration. From the experiment, the resulting optimum conditions are 23.88% (w/v) substrate loading, 109.43 U/g α-amylase concentration, 65.44 U/mL amyloglucosidase concentration and 74.87 rpm stroke speed, which yielded 196.23 g/L reducing sugar. The fermentation process was also carried out, with a production value of 0.45 g ethanol/g reducing sugar, which is equivalent to 88.61% of ethanol yield after fermentation by using Saccharomyces cerevisiae (S. cerevisiae). The physical and chemical properties of the produced ethanol are within the specifications of the ASTM D4806 standard. The good quality of ethanol produced from this study indicates that Manihot glaziovii (M. glaziovii) has great potential as bioethanol feedstock.

Record ID

LAPSE:2019.0441

Keywords

alternative fuel, bioethanol, Fermentation, hydrolysis, Manihot glaziovii (M. glaziovii), Optimization

Subject

Biosystems

Suggested Citation

Sebayang AH, Hassan MH, Ong HC, Dharma S, Silitonga AS, Kusumo F, Mahlia TMI, Bahar AH. Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology. (2019). LAPSE:2019.0441

Author Affiliations

Sebayang AH: Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia; Department of Mechanical Engineering, Medan State Polytechnic, Medan 20155, Indonesia
Hassan MH: Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia
Ong HC: Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia
Dharma S: Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia; Department of Mechanical Engineering, Medan State Polytechnic, Medan 20155, Indonesia
Silitonga AS: Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia; Department of Mechanical Engineering, Medan State Polytechnic, Medan 20155, Indonesia
Kusumo F: Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia
Mahlia TMI: Department of Mechanical Engineering, Universiti Tenaga Nasional, Kajang 43000, Selangor, Malaysia
Bahar AH: Department of Mechanical Engineering, Faculty of Engineering, The University of Melbourne, Parkville, VIC 3010, Australia
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Journal Name

Energies

Volume

10

Issue

1

Article Number

E35

Year

2017

Publication Date

2017-01-01