LAPSE:2018.0988
Published Article
LAPSE:2018.0988
Ethanol Production from Sweet Sorghum Juice at High Temperatures Using a Newly Isolated Thermotolerant Yeast Saccharomyces cerevisiae DBKKU Y-53
Sunan Nuanpeng, Sudarat Thanonkeo, Mamoru Yamada, Pornthap Thanonkeo
November 27, 2018
Ethanol production at elevated temperatures requires high potential thermotolerant ethanol-producing yeast. In this study, nine isolates of thermotolerant yeasts capable of growth and ethanol production at high temperatures were successfully isolated. Among these isolates, the newly isolated thermotolerant yeast strain, which was designated as Saccharomyces cerevisiae DBKKU Y-53, exhibited great potential for ethanol production from sweet sorghum juice (SSJ) at high temperatures. The maximum ethanol concentrations produced by this newly isolated thermotolerant yeast at 37 °C and 40 °C under the optimum cultural condition were 106.82 g·L−1 and 85.01 g·L−1, respectively, which are greater than values reported in the literatures. It should be noted from this study with SSJ at a sugar concentration of 250 g·L−1 and an initial pH of 5.5 without nitrogen supplementation can be used directly as substrate for ethanol production at high temperatures by thermotolerant yeast S. cerevisiae DBKKU Y-53. Gene expression analysis using real-time RT-PCR clearly indicated that growth and ethanol fermentation activities of the thermotolerant yeast S. cerevisiae DBKKU Y-53 at a high temperature (40 °C) were not only restricted to the expression of genes involved in the heat-shock response, but also to those genes involved in ATP production, trehalose and glycogen metabolism, and protein degradation processes were also involved.
Keywords
gene expression, high temperature fermentation, real-time RT-PCR, Saccharomyces cerevisiae, sweet sorghum, thermotolerant yeast
Subject
Suggested Citation
Nuanpeng S, Thanonkeo S, Yamada M, Thanonkeo P. Ethanol Production from Sweet Sorghum Juice at High Temperatures Using a Newly Isolated Thermotolerant Yeast Saccharomyces cerevisiae DBKKU Y-53. (2018). LAPSE:2018.0988
Author Affiliations
Nuanpeng S: Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand; Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
Thanonkeo S: Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham 44150, Thailand
Yamada M: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan
Thanonkeo P: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand; Fermantation Research Center for Value Added Agricultural Products, Khon Kaen University, Khon Kaen 40002, Thailand [ORCID]
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Journal Name
Energies
Volume
9
Issue
4
Article Number
E253
Year
2016
Publication Date
2016-03-31
Published Version
ISSN
1996-1073
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PII: en9040253, Publication Type: Journal Article
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LAPSE:2018.0988
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doi:10.3390/en9040253
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Nov 27, 2018
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CC BY 4.0
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Nov 27, 2018
 
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Calvin Tsay
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