This paper deals with the Differential Evolution (DE) based method for identification of the heat equation parameters applied for the estimation of a bare overhead conductor`s temperature. The parameters are determined in the optimization process using a dynamic model of the conductor; the measured environmental temperature, solar radiation and wind velocity; the current and temperature measured on the tested overhead conductor; and the DE, which is applied as the optimization tool. The main task of the DE is to minimise the difference between the measured and model-calculated conductor temperatures. The conductor model is relevant and suitable for the prediction of the conductor temperature, as the agreement between measured and model-calculated conductor temperatures is exceptional, where the deviation between mean and maximum measured and model-calculated conductor temperatures is less than 0.03 °C.