LAPSE:2018.0236
Published Article
LAPSE:2018.0236
Closed-Loop Characterization of Neuronal Activation Using Electrical Stimulation and Optical Imaging
July 31, 2018
We have developed a closed-loop, high-throughput system that applies electrical stimulation and optical recording to facilitate the rapid characterization of extracellular, stimulus-evoked neuronal activity. In our system, a microelectrode array delivers current pulses to a dissociated neuronal culture treated with a calcium-sensitive fluorescent dye; automated real-time image processing of high-speed digital video identifies the neuronal response; and an optimized search routine alters the applied stimulus to achieve a targeted response. Action potentials are detected by measuring the post-stimulus, calcium-sensitive fluorescence at the neuronal somata. The system controller performs directed searches within the strength⁻duration (SD) stimulus-parameter space to build probabilistic neuronal activation curves. This closed-loop system reduces the number of stimuli needed to estimate the activation curves when compared to the more commonly used open-loop approach. This reduction allows the closed-loop system to probe the stimulus regions of interest in the multi-parameter waveform space with increased resolution. A sigmoid model was fit to the stimulus-evoked activation data in both current (strength) and pulse width (duration) parameter slices through the waveform space. The two-dimensional analysis results in a set of probability isoclines corresponding to each neuron⁻electrode pair. An SD threshold model was then fit to the isocline data. We demonstrate that a closed-loop methodology applied to our imaging and micro-stimulation system enables the study of neuronal excitation across a large parameter space.
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Keywords
activation curve, closed-loop, dissociated culture, extracellular electrical stimulation, micro-electrode array (MEA), optical recording, strength-duration
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Suggested Citation
Kuykendal ML, Guvanasen GS, Potter SM, Grover MA, DeWeerth SP. Closed-Loop Characterization of Neuronal Activation Using Electrical Stimulation and Optical Imaging. (2018). LAPSE:2018.0236
Author Affiliations
Kuykendal ML: School of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA; Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA; Laboratory for Neuroenginee
Guvanasen GS: School of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA; Laboratory for Neuroengineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Potter SM: Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA; Laboratory for Neuroengineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Grover MA: School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA [ORCID]
DeWeerth SP: School of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA; Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA; Laboratory for Neuroenginee
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Guvanasen GS: School of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA; Laboratory for Neuroengineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Potter SM: Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA; Laboratory for Neuroengineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Grover MA: School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA [ORCID]
DeWeerth SP: School of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA; Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA; Laboratory for Neuroenginee
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Journal Name
Processes
Volume
5
Issue
2
Article Number
E30
Year
2017
Publication Date
2017-06-03
Published Version
ISSN
2227-9717
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Original Submission
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PII: pr5020030, Publication Type: Journal Article
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LAPSE:2018.0236
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doi:10.3390/pr5020030
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Jul 31, 2018
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